transam kits Search Results


transam sp1 kits  (Active Motif)
90
Active Motif transam sp1 kits
Transam Sp1 Kits, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dna-binding elisa-based transam kits  (Active Motif)
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Active Motif dna-binding elisa-based transam kits
Dna Binding Elisa Based Transam Kits, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transam transcription factor assay kits specific for p52  (Active Motif)
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Active Motif transam transcription factor assay kits specific for p52
Transam Transcription Factor Assay Kits Specific For P52, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa-based transam stat3/nfκb p65/ap-1 c-jun assay kits  (SciMed Asia)
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SciMed Asia elisa-based transam stat3/nfκb p65/ap-1 c-jun assay kits
Elisa Based Transam Stat3/Nfκb P65/Ap 1 C Jun Assay Kits, supplied by SciMed Asia, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nuclear extract and transam nfκb p65 transcription factor assay kit  (Active Motif)
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Active Motif nuclear extract and transam nfκb p65 transcription factor assay kit
Nuclear Extract And Transam Nfκb P65 Transcription Factor Assay Kit, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transam activity kits  (Active Motif)
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Active Motif transam activity kits
Transam Activity Kits, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nuclear extract transam nfat kits  (Active Motif)
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Active Motif nuclear extract transam nfat kits
Effect of MFN2 on intracellular calcium, calcineurin expression, and <t>NFAT</t> activity in Jurkat cells. Jurkat cells were transfected with LV-MFN2 and MFN2-RNAi and then stimulated with PMA (50 ng/ml) plus ionomycin (1 mmmol/L) for various lengths of time. (a and c) Intracellular calcium was measured by FACS with the fluorescent probe Fluo-3/AM. (b) Calcineurin activity was measured using a calcineurin assay kit. (d) NFAT activity in Jurkat cells was measured by ELISA. * P < 0.05, † P < 0.01 versus the control group; ‡ P < 0.05, § P < 0.01 versus the control-RNAi or LV-GFP group. LV: Lentiviral vector; MFN2: Mitofusin-2; GFP: Green fluorescent protein; NFAT: Nuclear factor of activated T cells; PMA: Phorbol myristate acetate.
Nuclear Extract Transam Nfat Kits, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transam tm ap-1/ phosphoc-jun enzyme-linked immunosorbant assay kit  (Active Motif)
90
Active Motif transam tm ap-1/ phosphoc-jun enzyme-linked immunosorbant assay kit
Effect of MFN2 on intracellular calcium, calcineurin expression, and <t>NFAT</t> activity in Jurkat cells. Jurkat cells were transfected with LV-MFN2 and MFN2-RNAi and then stimulated with PMA (50 ng/ml) plus ionomycin (1 mmmol/L) for various lengths of time. (a and c) Intracellular calcium was measured by FACS with the fluorescent probe Fluo-3/AM. (b) Calcineurin activity was measured using a calcineurin assay kit. (d) NFAT activity in Jurkat cells was measured by ELISA. * P < 0.05, † P < 0.01 versus the control group; ‡ P < 0.05, § P < 0.01 versus the control-RNAi or LV-GFP group. LV: Lentiviral vector; MFN2: Mitofusin-2; GFP: Green fluorescent protein; NFAT: Nuclear factor of activated T cells; PMA: Phorbol myristate acetate.
Transam Tm Ap 1/ Phosphoc Jun Enzyme Linked Immunosorbant Assay Kit, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transam kits cat#44096  (Active Motif)
90
Active Motif transam kits cat#44096
Effect of MFN2 on intracellular calcium, calcineurin expression, and <t>NFAT</t> activity in Jurkat cells. Jurkat cells were transfected with LV-MFN2 and MFN2-RNAi and then stimulated with PMA (50 ng/ml) plus ionomycin (1 mmmol/L) for various lengths of time. (a and c) Intracellular calcium was measured by FACS with the fluorescent probe Fluo-3/AM. (b) Calcineurin activity was measured using a calcineurin assay kit. (d) NFAT activity in Jurkat cells was measured by ELISA. * P < 0.05, † P < 0.01 versus the control group; ‡ P < 0.05, § P < 0.01 versus the control-RNAi or LV-GFP group. LV: Lentiviral vector; MFN2: Mitofusin-2; GFP: Green fluorescent protein; NFAT: Nuclear factor of activated T cells; PMA: Phorbol myristate acetate.
Transam Kits Cat#44096, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human erα transam™ kits  (Active Motif)
90
Active Motif human erα transam™ kits
Effect of MFN2 on intracellular calcium, calcineurin expression, and <t>NFAT</t> activity in Jurkat cells. Jurkat cells were transfected with LV-MFN2 and MFN2-RNAi and then stimulated with PMA (50 ng/ml) plus ionomycin (1 mmmol/L) for various lengths of time. (a and c) Intracellular calcium was measured by FACS with the fluorescent probe Fluo-3/AM. (b) Calcineurin activity was measured using a calcineurin assay kit. (d) NFAT activity in Jurkat cells was measured by ELISA. * P < 0.05, † P < 0.01 versus the control group; ‡ P < 0.05, § P < 0.01 versus the control-RNAi or LV-GFP group. LV: Lentiviral vector; MFN2: Mitofusin-2; GFP: Green fluorescent protein; NFAT: Nuclear factor of activated T cells; PMA: Phorbol myristate acetate.
Human Erα Transam™ Kits, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa kit ‘transam nfkb family kits  (Active Motif)
90
Active Motif elisa kit ‘transam nfkb family kits
Effect of MFN2 on intracellular calcium, calcineurin expression, and <t>NFAT</t> activity in Jurkat cells. Jurkat cells were transfected with LV-MFN2 and MFN2-RNAi and then stimulated with PMA (50 ng/ml) plus ionomycin (1 mmmol/L) for various lengths of time. (a and c) Intracellular calcium was measured by FACS with the fluorescent probe Fluo-3/AM. (b) Calcineurin activity was measured using a calcineurin assay kit. (d) NFAT activity in Jurkat cells was measured by ELISA. * P < 0.05, † P < 0.01 versus the control group; ‡ P < 0.05, § P < 0.01 versus the control-RNAi or LV-GFP group. LV: Lentiviral vector; MFN2: Mitofusin-2; GFP: Green fluorescent protein; NFAT: Nuclear factor of activated T cells; PMA: Phorbol myristate acetate.
Elisa Kit ‘Transam Nfkb Family Kits, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sp1 and sp3-specific transam kits  (Active Motif)
90
Active Motif sp1 and sp3-specific transam kits
Using an Sp-specific oligonucleotide-binding ELISA <t>(TransAm,</t> Active Motif), we measured the levels of <t>active</t> <t>Sp1</t> (Panel A) and Sp3 (Panel B) in the nucleus of mock transfected (Tat(−)) or pCP2-Tat101 transfected (Tat(+)) HPAEC. The signal was normalized to μg of nuclear protein. Mean ± SEM of biological replicates, n = 3, (***p ≤ 0.001 by unpaired student’s T-test).
Sp1 And Sp3 Specific Transam Kits, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Effect of MFN2 on intracellular calcium, calcineurin expression, and NFAT activity in Jurkat cells. Jurkat cells were transfected with LV-MFN2 and MFN2-RNAi and then stimulated with PMA (50 ng/ml) plus ionomycin (1 mmmol/L) for various lengths of time. (a and c) Intracellular calcium was measured by FACS with the fluorescent probe Fluo-3/AM. (b) Calcineurin activity was measured using a calcineurin assay kit. (d) NFAT activity in Jurkat cells was measured by ELISA. * P < 0.05, † P < 0.01 versus the control group; ‡ P < 0.05, § P < 0.01 versus the control-RNAi or LV-GFP group. LV: Lentiviral vector; MFN2: Mitofusin-2; GFP: Green fluorescent protein; NFAT: Nuclear factor of activated T cells; PMA: Phorbol myristate acetate.

Journal: Chinese Medical Journal

Article Title: Role of the Ca 2+ -Calcineurin-Nuclear Factor of Activated T cell Pathway in Mitofusin-2-Mediated Immune Function of Jurkat Cells

doi: 10.4103/0366-6999.223855

Figure Lengend Snippet: Effect of MFN2 on intracellular calcium, calcineurin expression, and NFAT activity in Jurkat cells. Jurkat cells were transfected with LV-MFN2 and MFN2-RNAi and then stimulated with PMA (50 ng/ml) plus ionomycin (1 mmmol/L) for various lengths of time. (a and c) Intracellular calcium was measured by FACS with the fluorescent probe Fluo-3/AM. (b) Calcineurin activity was measured using a calcineurin assay kit. (d) NFAT activity in Jurkat cells was measured by ELISA. * P < 0.05, † P < 0.01 versus the control group; ‡ P < 0.05, § P < 0.01 versus the control-RNAi or LV-GFP group. LV: Lentiviral vector; MFN2: Mitofusin-2; GFP: Green fluorescent protein; NFAT: Nuclear factor of activated T cells; PMA: Phorbol myristate acetate.

Article Snippet: Nuclear extract and TransAM NFAT kits were obtained from Active Motif (Carlsbad, CA, USA).

Techniques: Expressing, Activity Assay, Transfection, Enzyme-linked Immunosorbent Assay, Control, Plasmid Preparation

Regulation of calcineurin expression reverses MFN2-induced NFAT activation in Jurkat cells. After various pretreatments, Jurkat cells were stimulated with PMA plus ionomycin for 24 h. Jurkat cells were pretreated with MFN2-RNAi and then LV-calcineurin, and calcineurin activity was measured by commercial assay kit (a) and NFAT activity was determined by ELISA (c). Jurkat cells were pretreated with LV-MFN2 and then FK-506, and calcineurin activity (b) and NFAT activity (d) were measured. * P < 0.05 vs. the control group; † P < 0.05 vs. the control-RNAi or LV-GFP group; ‡ P < 0.05 vs. the LV-MFN2 or MFN2-RNAi group. LV: Lentiviral vector; MFN2: Mitofusin-2; GFP: Green fluorescent protein; NFAT: Nuclear factor of activated T-cell. P/I: PMA (phorbol myristate acetate)/ionomycin; ELISA: Enzyme-linked immunosorbent assay.

Journal: Chinese Medical Journal

Article Title: Role of the Ca 2+ -Calcineurin-Nuclear Factor of Activated T cell Pathway in Mitofusin-2-Mediated Immune Function of Jurkat Cells

doi: 10.4103/0366-6999.223855

Figure Lengend Snippet: Regulation of calcineurin expression reverses MFN2-induced NFAT activation in Jurkat cells. After various pretreatments, Jurkat cells were stimulated with PMA plus ionomycin for 24 h. Jurkat cells were pretreated with MFN2-RNAi and then LV-calcineurin, and calcineurin activity was measured by commercial assay kit (a) and NFAT activity was determined by ELISA (c). Jurkat cells were pretreated with LV-MFN2 and then FK-506, and calcineurin activity (b) and NFAT activity (d) were measured. * P < 0.05 vs. the control group; † P < 0.05 vs. the control-RNAi or LV-GFP group; ‡ P < 0.05 vs. the LV-MFN2 or MFN2-RNAi group. LV: Lentiviral vector; MFN2: Mitofusin-2; GFP: Green fluorescent protein; NFAT: Nuclear factor of activated T-cell. P/I: PMA (phorbol myristate acetate)/ionomycin; ELISA: Enzyme-linked immunosorbent assay.

Article Snippet: Nuclear extract and TransAM NFAT kits were obtained from Active Motif (Carlsbad, CA, USA).

Techniques: Expressing, Activation Assay, Activity Assay, Enzyme-linked Immunosorbent Assay, Control, Plasmid Preparation

Using an Sp-specific oligonucleotide-binding ELISA (TransAm, Active Motif), we measured the levels of active Sp1 (Panel A) and Sp3 (Panel B) in the nucleus of mock transfected (Tat(−)) or pCP2-Tat101 transfected (Tat(+)) HPAEC. The signal was normalized to μg of nuclear protein. Mean ± SEM of biological replicates, n = 3, (***p ≤ 0.001 by unpaired student’s T-test).

Journal: Free radical biology & medicine

Article Title: The HIV-Tat protein interacts with Sp3 transcription factor and inhibits its binding to a distal site of the sod2 promoter in human pulmonary artery endothelial cells

doi: 10.1016/j.freeradbiomed.2019.12.015

Figure Lengend Snippet: Using an Sp-specific oligonucleotide-binding ELISA (TransAm, Active Motif), we measured the levels of active Sp1 (Panel A) and Sp3 (Panel B) in the nucleus of mock transfected (Tat(−)) or pCP2-Tat101 transfected (Tat(+)) HPAEC. The signal was normalized to μg of nuclear protein. Mean ± SEM of biological replicates, n = 3, (***p ≤ 0.001 by unpaired student’s T-test).

Article Snippet: DNA-binding ELISAs for active Sp1 and Sp3 were carried out on nuclear extracts prepared as previously described [ 21 ] using Sp1 and Sp3-specific TransAM kits (Active Motif) according to manufacturer’s protocol.

Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Transfection